![]() The product needs to be protected from air, water and human contamination. It is difficult to develop and produce combination vaccines due to the possibility of incompatibilities and interactions among the antigens and other ingredients of the vaccines. The stabilizers increase the storage life, and preservatives allow the use of multi dose vials. The role of the adjuvant is to enhance the immune response of the antigen. Once the antigen is developed the vaccine is formulated by adding adjuvants, stabilizers, and preservatives. ![]() Recombinant proteins need many operations involving ultrafiltration and column chromatography for purification before they are ready for administration. For weakened or attenuated viruses no further purification may be required.
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